چکیده (انگلیسی):

Toxic outcome of chemical therapeutics as well as multidrug resistance are two serious phenomena for
their inacceptance in cancer chemotherapy. Antioxidants like curcumin (Cur) have gained immense
importance for their excellent anticarcinogenic activities and minimum toxic manifestations in biological
system. However, Cur is lipophilic and thus following oral administration hardly appears in blood indicating
its potential therapeutic challenge in cancer therapy. Nanocapsulated Cur has been used as a drug
delivery vector to focus the effectiveness of these vesicles against hepatocellular carcinoma. The theme of
work was to evaluate effectiveness in oral route of polylactide co-glycolide (PLGA) Nanocapsulated curcumin
(Nano Cur) against diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC) in rat.
Nano Cur of average diameter 14 nm and encapsulation efficiency of 78% were prepared. Fourier Transform
Infra Red (FTIR) analysis revealed that there is no chemical interaction between drug and the polymer.
Three i.p. injections of the chemical hepatocarcinogen DEN at 15 days interval causes hepatotoxicity,
the generation of reactive oxygen species (ROS), lipid peroxidation, decrease in plasma membrane microviscosity and depletion of antioxidant enzyme levels in liver. Nano Cur (weekly oral treatment for
16 weeks at 20 mg/kg b.wt) in DEN induced HCC rats exerted significant protection against HCC and
restored redox homeostasis in liver cells. Nanocapsulated Cur caused cancer cell apoptosis as visualized
by ApoBrdU analysis. Histopathological analysis confirmed the pathological improvement in the liver.
Nano Cur was found to be a potential formulation in oral route in combating the oxidative damage of
hepatic cells and eliminating DEN induced hepatocellular cancer cells in rat whereas identical amount
of free Cur treatment was found almost ineffective.