چکیده (انگلیسی):

Objective: To study the influence of curcumin on chemosensitivity of nephroblastoma
cells.
Methods: Human nephroblastoma cells line SK-NEP-1 was transplanted to the nude
mice subcutaneously to establish the implantation tumor model of human nephroblastoma
cells. A total of 30 tumor-bearing mice were divided into three groups of ten randomly.
The routine chemotherapy group was given vincristine (0.05 mg/mL$0.2 mL/d) and
actinomycin D (15 ng/mL$0.2 mL/d) combined chemotherapy regime. The curcumin
chemotherapy group was given the same combined chemotherapy regimens and curcumin
(30 mg/kg/d) by intraperitoneal injection. The control group was given normal saline
(NS) of the same volume by intraperitoneal injection. Continuous administration would
be kept for 4 weeks and 3 days a week. The volumetric changes of every group were
recorded. The serum of every group in different time was collected and the VEGF content
was detected by ELISA. All mice were cercrificed and the tumor tissues were stripped
and weighed after 4 weeks' treatment. The tumor inhibition rate was calculated. The cell
proliferation activity and apoptosis rate were detected by MTT and flow cytometry
method. All data were statistically analyzed by SPSS 19.0.
Results: The tumor volume, serum VEGF content, tumor inhibition rate, cell proliferation
activity and apoptosis rate of routine chemotherapy group and curcumin chemotherapy
group had significant differences comparing with the control group (P < 0.05)
after 4-week's treatment. The cancer growth of curcumin chemotherapy group was
obviously decreased and even tended to shrink comparing with routine chemotherapy
group (c2 = 15.732, P = 0.007). The cell proliferation activity was significantly reduced
and the apoptosis rate was significantly higher, (c2 = 9.427, P = 0.012) which showing
the effect of chemotherapy was enhanced.
Conclusions: The chemosensitivity of nephroblastoma cells could be improved by
curcumin, then the effect of preoperative adjuvant chemotherapy scheme would be
enhanced, the growth of nephroblastoma cells would be inhibited and the surgical risk of
nephroblastoma would be reduced.