چکیده (انگلیسی):

Objective: To study the effect of hGC-MSCs from human gastric cancer tissue on cell
proliferation, invasion and epithelial-mesenchymal transition in tumor tissue of gastric
cancer tumor-bearing mice.
Methods: BABL/c nude mice were selected as experimental animals and gastric cancer
tumor-bearing mice model were established by subcutaneous injection of gastric cancer
cells, randomly divided into different intervention groups. hGC-MSCs group were given
different amounts of gastric cancer cells for subcutaneous injection, PBS group was given
equal volume of PBS for subcutaneous injection. Then tumor tissue volume were
determined, tumor-bearing mice were killed and tumor tissues were collected, mRNA
expression of proliferation, invasion, EMT-related molecules were determined.
Results: 4, 8, 12, 16, 20 d after intervention, tumor tissue volume of hGC-MSCs group
were significantly higher than those of PBS group and the more the number of hGCMSCs,
the higher the tumor tissue volume; mRNA contents of Ki-67, PCNA, Bcl-2,
MMP-2, MMP-7, MMP-9, MMP-14, N-cadherin, vimentin, Snail and Twist in tumor
tissue of hGC-MSCs group were higher than those of PBS group, and mRNA contents of
Bax, TIMP1, TIMP2 and E-cadherin were lower than those of PBS group.
Conclusion: hGC-MSCs from human gastric cancer tissue can promote the tumor
growth in gastric cancer tumor-bearing mice, and the molecular mechanism includes
promoting cell proliferation, invasion and epithelial-mesenchymal transition