چکیده (انگلیسی):

Objective: To discuss the role of intestinal flora imbalance in the pathogenesis of
pouchitis.
Methods: The pouchitis rat model was established and the faeces sample and the mucous
membrane sample were collected regularly, in which the bacterial nucleic acids were
extracted for quantitative analysis of the intestinal flora in the samples through using the
real-time quantitative PCR technique and high energy sequencing technology.
Results: The disorder phenomenon of the intestinal flora appeared at the 7th day of the
experiment, and the pouchitis was presented at the 21st day of the experiment. At the 31st
day of the experiment, compared to control group and non-pouchitis group, the quantity
of Bifidobacterium and the Lactobacillus of the pouchitis model rats in the mucous
membrane sample and the faeces sample were significantly decreased (P < 0.05), and the
Bacteroidetes, Faecalibacterium prausnitzii and XIV Clostridium leptum subgroup in the
mucous membrane of pouchitis were significantly decreased (P < 0.05). The IV Clostridium
coccoides group was the main flora in the mucous membrane of pouchitis, the
bacterial diversity of non-pouchitis group and control group was significantly higher than
that of the pouchitis group (P < 0.05).
Conclusions: The intestinal flora imbalance is one of the factors that cause the incidence
of the pouchitis; this study provides a clue of the pathogenesis and treatment direction of
the intestinal inflammatory disease.