چکیده (انگلیسی):

Terbutaline sulfate (TBS) was assayed in biological samples by validated HPTLC
method. Densitometric analysis of TBS was carried out at 366 nm on precoated TLC aluminum
plates with silica gel 60F254 as a stationary phase and chloroform–methanol (9.0:1.0, v/v) as a
mobile phase. TBS was well resolved at RF 0.34 ± 0.02. In all matrices, the calibration curve
appeared linear (r2P 0.9943) in the tested range of 100–1000 ng spot1 with a limit of quantification
of 18.35 ng spot1. Drug recovery from biological fluids averaged P95.92%. In both matrices,
rapid degradation of drug favored and the T0.5 of drug ranged from 9.92 to 12.41 h at 4 C and from 6.31 to 9.13 h at 20 C. Frozen at 20 C, this drug was stable for at least 2 months (without
losses >10%). The maximum plasma concentration (Cpmax) was found to be 5875.03 ±
114 ng mL1, which is significantly higher than the maximum saliva concentration (Csmax,
1501.69 ±96 ng mL1). Therefore, the validated method could be used to carry out pharmacokinetic
studies of the TBS from novel drug delivery systems.