چکیده (انگلیسی):

Background: Cytomegalovirus (CMV) infection is one of the most common and severe
infections during the post-transplantation period. It threatens the survival of patients and the function
of the transplanted organ.
Aim of the study: To screen for CMV infection among solid organ transplantation patients using
monitoring of CMV phosphoprotein 65 (CMVpp65) antigenemia and to detect if CMV infection
and disease were associated with certain human leukocyte antigen (HLA)-A locus genotypes among
the studied group.
Subjects and methods: Thirty solid organ transplantation patients were included for posttransplantation
follow up for symptoms and signs suggestive of CMV disease upto one year. Regular
screening for CMV infection was done through CMVpp65 antigenemia detection by the
immunofluorescence technique. In addition, HLA-A genotype was determined for all patients using
the line probe assay.
Results: The present study showed that 9 out of 30 patients (30%) were positive for CMVpp65
antigenemia. The detected HLA-A alleles were HLA-A*01(no. = 16), HLA-A*02(no. =11),
HLA-A*11(no. = 5), HLA-A*19(no. =1), HLA-A*24(no. = 4), HLA-A*29(no. = 1),
HLA-A*30(no. = 16), HLA-A*92(no. = 4). Among the studied cases, 40% showed HLA-A*
01–30 type. There was a significant difference (P = 0.05) among detected HLA types as regards
CMVpp65 antigenemia, with HLA-A*02_11 representing 33.3% of CMVpp65 positive patients
and HLA-A*01_30 representing 57.1% of CMVpp65 negative patients.