چکیده (انگلیسی):

This work aims at optimizing the high cell density fungal cultivation for producing large quantities of
fungal biomass to be used in azo dye residues bioremediation. In our previous studies the efficacy of using
certain fungal strains to decolorize a range of commercial textile dyes of different structures (azo, disazo)
were investigated. Several promising fungal strains belonging to Aspergillus tubigenesis,Aspergillus niger,
Aspergillus terreus, and Aspergillus fumigates demonstrated high capacity in decolorizing various azo dyes.
This study focuses on the high cell density cultivation of the fungal strains identified as potential
bioremediation agents. The study includes the optimization of all parameters involved in bioprocess
development for high cell density cultivation of six promising fungal strains. The growth of the fungal
strains was tested on the sucrose medium in 7 l-fermenter. The growth of these fungal strains having the
capacity to accumulate large quantities of biomass was also tested in medium containing molasses as a
cheap substrate. The residual molasses, biomass dry weight and protein content of the six fungal strains
showed that the strains 20 and 2 were marked by the highest protein content. In this study a comparative
analysis between the results of dry weight, residual molasses and protein content of geowth of the strains
20, 5 and 2 under uncontrolled and controlled pH of media in batch fermentation was studied to follow
the accumulation of biomass and protein production in the growth media. The results indicate that the
dry weight accumulated by strains No. 20, 5 and 2 grown on molasses was better than those of strains
grown on sucrose. Fungal strain No. 5 had the highest biomass dry weight accumulation. The study shows
that the molasses as cheaper sugar sources were better than sucrose for growing fungal biomass.
ã 2016 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license