چکیده (انگلیسی):

Background: Single nucleotide polymorphism (SNP) within tumor necrosis factor alpha
(TNF-a) gene promoter (308G/A TNFA) is associated with higher gene expression. The role of
this SNP as a risk factor for spontaneous preterm birth has been assessed in some regions and
the findings were significantly different between race and ethnic groups.
Aim: To provide the scientific evidence whether allele A within SNP 308G/A TNFA promoter
is a risk factor for spontaneous preterm birth among Acehnese ethnic or not.
Subjects and methods: In this case–control study, the genotypes of SNP 308G/A TNFA among
40 patients with spontaneous preterm birth and 40 patients with term birth were determined by realtime
polymerase chain reaction (RT-PCR). The concentrations of TNF-a from blood were measured
by enzyme-linked immunosorbent assay (ELISA). The differences in genotype distributions,
dominant and recessive models, and allele frequencies between case and control groups were analyzed
with Chi-squared test. Deviation of genotype frequencies from the Hardy–Weinberg equilibrium
(HWE) was assessed by Fisher’s exact test.
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