چکیده (انگلیسی):

Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis
mediterranei (NCH) was carried out. The use of aeration level 1.5 vvm increased the yield
by 16.6% (from 13.81 to 16.1 g/l) upon controlling the temperature at 28 C. Adjustment of the aeration
level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO) at 30% saturation further
increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a
production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH
at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling
the temperature at 30 C for 3 days then 28 C thereafter slightly increased the yield by 5%
upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm.
Integration of the most optimum conditions increased the production yield by 22% from 13.81
to 17.8 g/l.