چکیده (انگلیسی):

Objective: To observe the effects of perfusion of the gastrodin in abdominal aorta for
alleviating the spinal cord ischemia reperfusion injury (SCIRI).
Methods: A total of 36 New Zealand white rabbits were divided randomly into shamoperated
group (group S), control group (group C) and gastrodin group (group G), 12
rabbits for each group. Aorta abdominalis infrarenalis blocking method was applied to
establish the SCIRI model. The changes of motor evoked potentials (MEPs) before the
ischemia and on 30 min, 60 min, 6 h, 12 h and 24 h of reperfusion of the gastrodin were
respectively recorded, and the neurologic function score before the ischemia, on the 6 h,
12 h and 24 h of the reperfusion of the gastrodin were assessed. And the changes of the
concentration of serum neuron specific enolase (NSE), interleukin (IL)-lb and IL-8 were
measured before the ischemia, after 45 min of ischemia, and on 30 min, 60 min, 6 h, 12 h
and 24 h of reperfusion of gastrodin. Then the levels of spinal cord nerve cells mitochondrial
superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione
peroxidase (GSH-PX), malondialdehyde (MDA), total antioxidant capacity (T-AOC) and
mitochondrial swelling degree (MSD) were tested and the histopathologic changes in
spinal cord tissues were observed.
Results: The levels of the NSE, IL-lb, IL-8, ROS, MDA and MSD of group C were all
significantly elevated after the ischemia (P < 0.01); the levels of the spinal nerve cell
mitochondria SOD, GSH-PX and T-AOC were all significantly reduced (P < 0.01),
MEPs and spinal cord tissue pathology were damaged significantly (P < 0.01). The rate
of motor neuron abnormalities and the damages of spinal cord tissue pathology of group
G were significantly milder than those of group C (P < 0.01); the levels of NSE, IL-lb,
IL-8, ROS, MDA and MSD were significantly lower than those of group C (P < 0.01),
but the levels of SOD, GSH-PX and T-AOC were all significantly higher than those of
group C (P < 0.01), and the recovery of neurologic function score during the reperfusion
of gastrodin was significantly faster than group C (P < 0.01).
Conclusions: Perfusion of the gastrodin in abdominal aorta can alleviate the spinal cord
ischemia reperfusion injury by promoting the mitochondrial antioxidant capacity and
inhibiting the inflammatory reaction.