چکیده (انگلیسی):

Objective: To assess the protective potential of a-tocopherol on ethanol-induced dysmorphogenesis
in 10.5 embryonic day (ED) mouse embryos.
Methods: Forty female mice were randomly assigned into control (CON), positive control
(ETOH), low-, medium and high-a-tocopherol-supplemented-Ethanol groups (LTOC,
MTOC,HTOC, respectively). CONreceived drinkingwater without ethanol, ETOH, LTOC,
MTOCandHTOC groups received 20%ethanol in drinkingwater. The supplemented groups
were given respective dosages of a-tocopherol, 0.410, 0.819 and 1.640mg/g body weight, at
14 days before mating until the 9th day of gestation. The 10.5 ED embryos were assessed for
embryo weight, head- and crown-rump length, and morphological scoring of brain and
sensory vesicles, flexion and somites. The embryo yield was assessed by counting the
number of full-term developed embryos from the bulging implantation sites while resorption
was assessed by counting the bulging implantation sites but without formed embryos.
Results: The weight and head- and crown-rump length of the embryos from the atocopherol
supplemented groups were comparable to the control. These were significantly
higher than that of positive control (P < 0.05). Overall morphological scores of the hindbrain
and sensory vesicles were significantly higher in the supplemented and control groups
than that of the positive control (P < 0.05). The number of full-term developed embryos was
neither affected by ethanol alone nor with supplementation with a-tocopherol. Resorption
was significantly lower in the supplemented groups than that of positive control (P < 0.05).
Conclusion: The medium and high dosages of a-tocopherol exhibited a protective effect
on ethanol-induced dysmorphogenesis.