چکیده (انگلیسی):

Metabolomic profiling was carried out on 53 post-mortem brain samples from subjects diagnosed with schizophrenia,
depression, bipolar disorder (SDB), diabetes, and controls. Chromatography on a ZICpHILIC column
was used with detection by Orbitrap mass spectrometry. Data extraction was carried out with m/z Mine 2.14
with metabolite searching against an in-house database. There was no clear discrimination between the controls
and the SDB samples on the basis of a principal components analysis (PCA) model of 755 identified or putatively
identified metabolites. Orthogonal partial least square discriminant analysis (OPLSDA) produced clear separation
between 17 of the controls and 19 of the SDB samples (R2CUM 0.976, Q2 0.671, p-value of the cross-validated
ANOVA score 0.0024). The most important metabolites producing discrimination were the lipophilic amino
acids leucine/isoleucine, proline, methionine, phenylalanine, and tyrosine; the neurotransmitters GABA and
NAAG and sugar metabolites sorbitol, gluconic acid, xylitol, ribitol, arabinotol, and erythritol. Eight samples
from diabetic brains were analysed, six of which grouped with the SDB samples without compromising the
model (R2 CUM 0.850, Q2 CUM 0.534, p-value for cross-validated ANOVA score 0.00087). There appears on
the basis of this small sample set to be some commonality between metabolic perturbations resulting from
diabetes and from SDB.