چکیده (انگلیسی):

Objective: To prove that the decrease in the expression of protamine 2 affects the
fertilization ability and the number of child production.
Methods: During the development stage of elongating spermatids, human spermatozoa
chromatin undergoes a complex transition where histone is extensively replaced by
protamine. Histone substitution by protamine 1 and 2 plays a significant role in
condensing chromatin required to induce the quality of potent spermatozoa. The substitution
also has a key role in protecting spermatozoa from effects of free radicals which
can degrade the spermatozoa quality.
Results: The results revealed that protamine deficiency instigated a severe disruption of
spermatogenesis affecting male infertility. In addition, the protamine expression disorder
caused a decrease in number, motility, and morphology of spermatozoa.
Conclusion: Our results of a study in mice confirmed that inhibin B injection caused a
reduction in the expression of protamine 2 in cauda epididymis. It has implications in the
decrease of motility, concentration, and spermatozoa viability so that it affects the
fertilization ability and the number of child production.