چکیده (انگلیسی):

Objective: To examine effects of total green tea extract, a potent free radical scavenger
on testicular tissue oxidative status.
Methods: 32 male albino rats of Wistar strain were divided into four groups, every group
restricted 8 animals: (i) control rats; (ii) green tea-treated control rats; (iii) malathion rats;
(iv) malathion-treated green tea rats. Animals received malathion 150 mg/kg and green
tea 30 mg/kg for 24 h intraperitoneally. At the end of the treatment period, rat testis
tissues were quickly removed and analyzed. Diameter of seminiferous tubules and
germinal cell thickness, spermatogonia sertoli cells, primary spermatocytes, spermatids
and leydig cell were evaluated. Also, oxidative stress evaluation was conducted based on
total antioxidant capacity (TAC), lipid peroxidation (LPO) and total thiol molecules
(TTM) in homogenate testis tissues.
Results: The results showed that total green tea extract improve oxidative damages
against malathion group. Light photomicrograph of seminiferous tubules in malathiontreated
group showed noticeable reduced height of germinal epithelium and disorganization
of the tubules. An increased intestinal tissue was also observed. Primary
spermatocytes were located distance from basal lamina indicating it induced damages to
the intestinal tissue. While seminiferous tubules in malathion exposed and green tea
extract-treated were normal.
Conclusion: This study demonstrated the effectiveness of TGTE on oxidative stress and
testicular tissue damage induced in malathion in infertility disorders.