چکیده (انگلیسی):

Objective: To identify the influence on radiosensitivity of lung glandular cancer cells
when excisions repair cross-complementing group1 (ERCC1) gene was silenced by targeted
siRNA.
Methods: siRNA which targeting to ERCC1 and control siRNA was designed and
synthesized. The human lung glandular cancer SPC-A-1 cells was transfected. A total of
56 nude mice were divided into two groups, and two kinds of SPC-A-1 cells were
transplanted to armpit of right forelimb, to establish the nude mice subcutaneous xenotransplanted
tumor model of human lung glandular cancer cells. After the tumor was
developed, the nude mice were randomly divided into four groups and accepted different
doses of X-Ray radiation, then the change of tumor volume, survival time of mice in
every group were recorded and the average lifetime was calculated. Twenty-one days
later of X-ray experiment, two mice were taken and killed in each group and the tumors
organizations were stripped. The cell apoptosis rate and cell cycle distributions were
obtained by FCM (flow cytometry).
Results: The volume of tumor which ERCC1 gene was silenced was less than single
irradiation group after X-ray irradiation, and the growth speed was slower and the lifetime
of mice was lengthened as well (P < 0.05). The cells apoptosis rate and the rate of G2/M
cells which ERCC1 gene was silenced were higher than the same dose control group and
the rate of G1 cells were lower, which indicated that the cells could be stopped at G2/M
point, the cell proliferation was inhibited, the cell apoptosis was promoted and the radiation
sensitivity was improved after the ERCC1 was silenced.
Conclusions: The radiation sensitivity of lung glandular tumor could be improved after
the ERCC1 gene was silenced by siRNA.