چکیده (انگلیسی):

Objective: To evaluate the effect of skim milk and/or coconut milk in a glycerol-free
trehalose extender to improve cryosurvival of boar spermatozoa.
Methods: Sperm samples were diluted in an egg-yolk-based freezing extender containing
100 mM trehalose and 0.25% Equex STM supplemented with coconut milk or/
and skim milk at 2% or 5% (w/v). Spermatozoa were cryopreserved in 0.5 mL straws and
thawed by a rapid transient method (at 70
C for 8 s) followed with a stabilizing procedure
at 39
C. Thawed samples were analyzed for motility, viability, high mitochondrial
membrane potential (HMMP), and acrosome damage.
Results: Even on the presence of egg yolk, motility, HMMP and viability were significantly
higher in extender supplemented with 2% skim milk than controls without skim
milk (P < 0.05). Post-thaw viability significantly improved with the addition of 2% skim
milk plus 2% coconut milk as well (P < 0.05). Acrosome damage was considerably lower
when the extender was supplemented with 2% coconut milk (P < 0.05), whereas the
benefit was masked in the presence of 2% skim milk.
Conclusion: 2% skim milk can be used as supplements for a glycerol-free trehalose and
egg yolk-based extender to improve post-thaw survival of boar spermatozoa, whereas 2%
coconut milk has an effect to protect boar spermatozoa from acrosome damage.