چکیده (انگلیسی):

Objective: To examine the multiplication efficiency Japanese encephalitis virus (JEV)
genotype I (GI) and genotype III (GIII) of different cell lines which originated from
human, porcine, mosquitoes in order to prove mechanism of JEV GI replacement JEV
GIII since it emerging in nature recent decades.
Methods: The mixture of GI and GIII JEV isolates was inoculated on human rhabdomyosarcoma
(RD), pig kidney epithelial (PS) and Aedes albopictus C6/36 clone (C6/36)
which originated from human, porcine and mosquitoes, respectively. Plaque assays were
performed to calculate virus titer and real-time RT-PCR with GI and GIII specific primer
sets to quantify the number of GI and GIII RNA copies.
Results: The highest virus titer reached at the 3rd day of post infection when GI and GIII
mixture was inoculated on RD and PS and that of C6/36 was at the 4th day. JEVs were
amplified and maintained by C6/36 cells after 10 passages whereas that by RD and PS
only limited within 8 and 6 passages, respectively. GI strain amplified and maintained
more efficiently on C6/36 and PS but not RD, whereas GIII strain amplified and maintained
more efficiently on RD.
Conclusions: There is a correlation between the multiplication efficiency of GI and GIII
JEV strains when these two genotype strains co-infected on different cell lines with the
predominance of GI strains in C6/36 and PS and the limited detection of GI strains in RD
cells proving a possible mechanism of shift JEV genotypes in nature recent decades since
GI emerging.