چکیده (انگلیسی):

Objective: To detect the expression of Toll-like receptor 4 (TLR-4) and NF-kB and to
discuss the mechanism of TLR-4/NF-kB pathway in the myocardial ischemia reperfusion
injury of mouse.
Methods: TLR-4 mutant mice and wild homozygous mice were divided into the model
group and sham group. Mice in the model group were given the ligation of left anterior
descending coronary artery for the modeling, while mice in the sham group were not
given the ligation after threading. The cardiac muscle tissues were collected for the
morphological observation. The immunohistochemistry was employed to detect the
expression of NF-kB, Western blot was used to detect the expression of TLR-4 and
ELISA to detect the expression of serum inflammatory factors.
Results: The expression of NF-kB in TLR-4 null mice after the myocardial ischemia
reperfusion was significantly lower than that in wild homozygous mice. For the model
group and sham group, the expression of TLR-4 in wild homozygous mice was all
significantly higher than that in TLR-4 null mice, while the expression of TLR-4 in TLR-4
null mice in the model group was significantly higher than that in sham group, with the
statistical difference (P < 0.05). The expression of inflammatory factors in TLR-4 null mice
and wild homozygous mice in the model group was significantly higher than that in sham
group. The expression of all factors in group A with TLR-4 null was significantly lower
than that in group B with wild homozygous type, with the statistical difference (P < 0.05).
Conclusions: TLR-4/NF-kB pathway is closely related to the myocardial ischemia
reperfusion injury, which plays its role through the release of inflammatory cytokines.