چکیده (انگلیسی):

Objective: To discuss the expression of long noncoding RNA TUG1 (lncRNA-TUG1)
in gastric carcinoma (GC) and its effects on the transferring and invading capacity of
gastric carcinoma cells.
Methods: Forty cases of carcinoma tissue and para-carcinoma tissue were selected from
GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese
Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December,
2014; the expressing level of lncRNA-TUG1 in GC and para-C tissues was detected by
applying the qRT-PCR technique. The correlation between lncRNA-TUG1 expression
and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected
using lncRNA-TUG1 specific siRNA. Changes of the transferring and invading capacity
of siRNA-transfected SGC-7901 cells were scratch-tested and transwell-detected. qRTPCR
was applied to detect the expression level of microRNA-144 after lncRNA-TUG1
was silenced. Changes of c-Met mRNA and protein expressions was detected by qRTPCR
and western-blot test.
Results: The expression level of lncRNA-TUG1 in GC tissue was significant higher than
that in para-C tissue (P < 0.05) and the high expression level of lncRNA-TUG1 in GC
tissue was significantly correlated with tumor lymph nodes metastasis and advance TNM
phasing (P < 0.05). The transferring and invading capacity of SGC-7901 cells was highly
inhibited after being transfected by lncRNA-TUG1 specific siRNA (P < 0.05). The results
of qRT-PCR and western-blot proved that the expression of microRNA-144 was
significantly boosted and the expression level of c-Met mRNA and protein was inhibited
after lncRNA-TUG1 was silenced (P < 0.05).
Conclusions: lncRNA-TUG1 shows an up-regulated expression in GC tissue and that
bears a correlation with clinicopathological features of malignant tumor. lncRNA-TUG1
can promote the transferring and invading capacity of GC by inhibiting the pathway of
microRNA-144/c-Met.