چکیده (انگلیسی):

Objective: To investigate the gene related to b-lactam resistance and to confirm the
mechanism about a synergy effect between CPZ and b-lactam antibiotics.
Methods: To measure antibacterial activity, we performed a minimum inhibitory concentration
(MIC) and synergy test. Transmission electron microscopy (TEM) was used in
morphological analysis. To analyze gene expression, we conducted reverse transcriptase
polymerase chain reaction (PCR).
Results: We confirmed a synergy effect between CPZ and b-lactam antibiotics.
Furthermore, we observed that CPZ affect the cell envelope of MRSA by using TEM. At
the gene level, CPZ reduced the expression of resistance genes.
Conclusions: Through this result, we hypothesize that a decrease of resistance factor
expressions was caused by CPZ because it disrupts the activity of a sensor protein located
in the cell membrane.