چکیده (انگلیسی):

Objective: To study the inhibition effect of miR-106a inhibitor on tumor growth of
ovarian cancer xenografts mice.
Methods: BALB/c mice were selected as experimental animals, ovarian cancer SKOV-3
cells transfected with miR-106a inhibitor and its negative control were inoculated subcutaneously,
intratumoral injection of miR-106a inhibitor and its negative control were
continued after tumor formation, and they were enrolled as treatment group and model
group, respectively. Tumor volume and weight as well as Ki-67 and programmed cell
death 4 (PDCD4) expression were determined; miR-106a inhibitor and its negative
control as well as miR-106a mimic and its negative control were transfected into SKOV-3
cells, and expression of PDCD4 in cells was determined.
Results: Tumor tissue volume and weight as well as mRNA expression and protein
expression of Ki-67 in treatment group were significantly lower than those in the model
group while mRNA expression and protein expression of PDCD4 were significantly
higher than those in the model group; transfection of miR-106a mimic could decrease
mRNA expression and protein expression of PDCD4 in SKOV-3 cells, and transfection
of miR-106a inhibitor could increase mRNA expression and protein expression of
PDCD4 in SKOV-3 cells.
Conclusions: Transfection of miR-106a inhibitor can inhibit the growth of tumor in
ovarian cancer xenografts mice through increasing the expression of PDCD4.