چکیده (انگلیسی):

Objective: To study the therapeutic mechanisms of pseudolaric acid on allergic contact
dermatitis in mice.
Methods: A total of 50 BALB/C mice were selected and randomly divided into control
group, model group, and treatment A, B, C groups with 10 rats in each group. ACD model
was established inmodel group, and treatment A, B,Cgroups but not in control group. Model
group received no treatment, but treatment A, B, C groups were treated with external
application of the concentration of 0.1%, 0.2% and 0.4% of the pseudolaric acid for the lesions
of ear skin. And the weight gain and the swelling degree of the mice' ear were recorded,
weight of thymus and spleen were measured. Spleen suspension was prepared to test T
lymphocyte and B lymphocyte levels of mice in five groups. Changes in serum IFN-g, IL-4
and IL-10 levels were tested through the enzyme linked immunosorbent assay (ELISA).
Results: The weight gain of mice in model group were significant lower than those of
mice in the control group and the treatment A, B, C groups (P < 0.05). Weight gain of
mice in treatment A, B groups were significant lower than that of control group
(P < 0.05), but the difference in weight gain between treatment C group and control
group showed no significant difference (P > 0.05). The swelling degree and the weight of
mice ears in model group were significant higher than those of mice in control group and
treatment A, B, C groups (P < 0.05). Swelling degree and the weight of mice ears of
treatment A, B, C groups were obviously higher than that of control group (P < 0.05).
The swelling degree and weight of mice' ears in treatment A, B, C groups were decreased
with the increase of the drug dosage, but comparison between A, B and C group showed
statistically differences (P < 0.05). The thymus and spleen index of mice in model group
were significant higher than those of the other four groups (P < 0.05), among the four
groups, thymus and spleen index of treatment A and B group were higher than control
group and treatment C group (P < 0.05). The stimulation index of T and B cells of mice
in model group was significantly higher than the rest four groups (P < 0.05). The serum
IFN-g level of mice in control group and treatment A, B and C group was obviously
lower than that of mice in model group (P < 0.05). The serum IFN-g level of mice in
treatment A, B and C group were decreased with the increasing of the drug dosage, and
the level of C group was obviously lower than that of A and B group (P < 0.05).
Conclusion: The pseudolaric acid has anti-inflammation and immune adjustment the
effects showing a remarkable therapeutic effects for the ACD mice.