چکیده (انگلیسی):

Objectives: To evaluate possible lipid catabolism and body fat regulation effects of 3-
caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-
induced obese mice.
Methods: Obesity was induced in mice using a HFD for four weeks. Then, mice were
fed only HFD or HFD with GCBE at 50, 100, and 200 mg/kg. Fatty acid synthesis
mechanism regulation of body fat was investigated through real-time PCR and Western
blot assay. Body fat reduction was measured through dual-energy X-ray absorptiometry.
Results: In HFD-induced obese mice, GCBE treatment significantly decreased body
weight gain, liver weight and white adipose tissue weights with regulation of adipose
tissue lipolysis hormones, like adiponectin and leptin. GCBE treatment decreased mRNA
expression levels of adipogenesis and adipocyte metabolism related genes in adipose
tissues and the liver, and decreased the corresponding protein expression. Dual energy Xray
absorptiometry measurements were used to compare body fat between mice on highfat
and those treated with GCBE. GCBE treated mice had a lower fat mass compared to
HFD alone fed mice and relative body weight and fat mass were markedly decreased.
Conclusions: GCBE has a potential anti-obesity effect with lowering body fat accumulation
by regulating adipogenesis and lipid metabolism-related genes and proteins in
WAT and liver