چکیده (انگلیسی):

An attempt has been made to isolate potent amylase producing gut bacteria from
Gryllotalpa africana. Out of 82 isolates, GAA 31.1 was selected as potent producer, having enzyme
activity 9.6± 0.861 U/ml. The isolate GAA 31.1 was identified as Rhodococcus opacus following
morphological, biochemical, physiological characterization and phylogenetic analysis through
16S rRNA gene sequencing. Fatty acid methyl ester profile of the isolate was also studied. The
optimized physical cultural conditions for amylase production were found as incubation period
48 h, inoculum volume 2%, initial pH of the fermentation medium 7.0, temperature 38
C and
aeration at 150 rpm. Optimum nutrient conditions were determined as: supplementation of maltose
1.4% and sodium nitrate 1.4%. Surfactants SDS, EDTA, Tween 80 and Triton X-100 showed positive
effect on enzyme production. Riboflavin (50 lg/ml) among the tested vitamins stimulated the
production maximally. The isolate was also able to produce amylase using agro-industrial waste.
This actinobacterium may be a potent candidate for amylase as it is capable of enhanced production
(326.72 ± 6.081 U/ml) by utilizing agro-residues.
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Technology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/
licenses/by-nc-nd/4.0/).