چکیده (انگلیسی):

Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated
from Assam located in North East region of India. Morphological identification of Fusarium
isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates
was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal
DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity.
Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40
primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the
relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an
indispensable method for identification studies.

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