چکیده (انگلیسی):

Objective: To discuss the liver function damage mechanism of patients with clonorchiasis
by analyzing the ultrasound characteristics, liver function, change of the serum inflammatory
factors and cell apoptosis factors.
Methods: Color Doppler ultrasound technique was adopted to detect the portal vein and
blood flow change of patients with clonorchiasis; ELISA was used to determine the level of
different serum inflammatory factors. The levels of serum total bilirubin, serum albumin and
glutamic-pyruvic transaminase were detected by automatic biochemical analyzer. Western
blot was used to determine the expression of proteins relevant to apoptosis.
Results: Compared with the health control group, the trunk diameter of portal vein and
the thickness of spleen, as well as the hepatic artery pulsation index of clonorchiasis
patients increased obviously, the mean blood flow velocity of portal vein (P < 0.05 or
P < 0.01) decreased. The content of total bilirubin and transaminase in plasma increased
significantly, but albumin decreased (P < 0.05). Levels of TNF-a, IL-6 and IFN-g
increased remarkably, and the level of every factor was significantly different among
patients with Child-Pugh , Child-Pugh II and Child-Pugh III classification of liver
function (P < 0.05 or P < 0.01). With the exacerbation of liver dysfunction, levels of
TNF-a, IL-6 and IFN-g gradually increased (P < 0.05). Compared with the healthy
control group, the expression quantity of apoptosis protein Fas, FasL, Bax and Caspase-3
increased significantly (P < 0.05 or P < 0.01), but Bcl-2 decreased (P < 0.05).
Conclusions: Changes of ultrasonic characteristics and liver dysfunction, caused by liver
fluke infection, may be related to that both inflammatory response and apoptosis response
have participated in the pathogenic process and liver damage course of clonorchiasis.