چکیده (انگلیسی):

Maize is one of the important cereal crops around the world. An efficient and reproducible
regeneration protocol via direct organogenesis has been established using split nodes as
ex-plants derived from 7 to 10 day old in vitro grown seedlings. Surface sterilized maize seeds were
germinated on MS medium lacking plant growth regulators. Nodal sections of 7–10 day old
seedlings were isolated, split longitudinally into two halves and cultured on regeneration medium
containing different concentrations of 6-benzyladenine (2.20, 4.40, 6.60, 8.80, 11.0 and 13.2 lM)
or kinetin (2.32, 4.65, 6.97, 9.29, 11.6 and 13.9 lM). Inclusion of 8.80 lM BA into MS supplemented
medium triggered a high frequency of regeneration response from split node explants with
a maximum number of shoots (12.0 ±1.15) and the highest shoot length (3.0 ± 0.73) was obtained
directly (without an intervening callus phase) within 4 weeks of culture. Further shoot elongation
was achieved on medium containing 4.40 lM BA. The elongated micro shoots were rooted on
MS medium fortified with 1.97 lM indole-3-butyric acid. The regenerated plantlets with roots were
successfully hardened on earthen pots after proper acclimatization under greenhouse conditions.
This new efficient regeneration method provides a solid foundation for genetic manipulation of
maize for biotic and abiotic stresses and to enhance the nutritional values.

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