چکیده (انگلیسی):

Background: The chemokine receptor, CC-chemokine receptor 3 (CCR3), and its major ligands, eotaxin,
RANTES, and MCP-4, are involved in eosinophil chemotaxis. It is thought that CCR3 plays an important
role in the recruitment and activation of eosinophils in nasal polyposis. We examined nasal polyp
extract-induced eosinophil chemotaxis and the effect of a CCR3 antagonist using EZ-TAXIScan, a novel
real-time chemotaxis assay device.
Methods: Nasal polyps were obtained from chronic rhinosinusitis (CRS) patients during surgery. The
polyps were homogenized and eotaxin levels in the extracts were measured. Eosinophils were purified
from human peripheral blood by the CD16 negative selection method. Nasal polyp extract-induced
eosinophil chemotaxis, with or without CCR3 antagonist, was assessed by EZ-TAXIScan.
Results: There was a significant positive correlation between the eosinophil counts in nasal polyp and
eotaxin levels in the nasal polyp extracts. Using EZ-TAXIScan, eosinophil chemotactic responses were
observed following stimulation with nasal polyp extracts. There was a significant positive correlation
between the chemotactic index toward the nasal polyp extracts and their eotaxin levels. Nasal polyp
extract-induced chemotaxis was completely inhibited by CCR3 antagonist but not by chemoattractant
receptor-homologous molecule expressed on Th2 cells (CRTH2) antagonist which inhibited PGD2-
induced eosinophil chemotaxis.
Conclusions: The CCR3 pathway may play an important role in the pathogenesis of eosinophil recruitment
in nasal polyps through selective eosinophil chemotaxis.