چکیده (انگلیسی):

Two simple, accurate and sensitive and techniques have been developed and validated for
the determination of yohimbine hydrochloride in presence of its acid, alkaline and photo-degradates
as stability-indicating studies. First spectrophotometric technique was adopted for the determination
of the investigated drug in presence of degradation products, by the use of derivative and derivative
ratio methods, respectively, while the second technique utilized a high-performance liquid chromatography
for the determination of yohimbine hydrochloride in presence of its degradation products, as stability
indicating method. The chromatographic separation was achieved isocratically by using a mobile
phase of water and methanol in a ratio of 55:45 V/V containing 0.5% triethylamine. The analysis was
carried out using C18 (4.6 · 150 mm, 5 lm) at flow rate of 1.0 ml/min and UV detection at 270 nm. All
the proposed methods were validated according to the International Conference on Harmonization
(ICH) guidelines and successfully applied for determination of the drug in pure form, in laboratory prepared
mixtures and in pharmaceutical preparations. The obtained results were statistically compared to
the official method of analysis for yohimbine hydrochloride and no significant differences were found.