چکیده (انگلیسی):

This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLsproducing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces inIran were screened for resistance to extended-spectrum cephalosporins. After identificationand susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evalu-ated for ESBL production by the disk combination method and by Etest using (cefotaxime andcefotaxime plus clavulanic acid). All isolates were also screened for blaCTX-Musing conven-tional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonellaspp and Shigella spp isolates were MDR, respectively. The presence of CTX-M enzyme amongESBL-producing isolates was 85.18%, 77.7%, 50%, and 66.7%, in E. coli, Klebsiella spp, Salmonellaspp and Shhttp://irarticles.ir/wp-admin/post-new.php?post_type=download#igella spp respectively. The overall presence of CTX-M genes in Enterobacteriaceaewas 15.4% and among the resistant isolates was 47.6%. This study indicated that resistanceto -lactams mediated by CTX-M enzymes in Iran had similar pattern as in other parts ofthe world. In order to control the spread of resistance, comprehensive studies and programsare needed.© 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Ma b s t r a c tThis study was conducted in Iran in order to assess the distribution of CTX-M type ESBLsproducing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces inIran were screened for resistance to extended-spectrum cephalosporins. After identificationand susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evalu-ated for ESBL production by the disk combination method and by Etest using (cefotaxime andcefotaxime plus clavulanic acid). All isolates were also screened for blaCTX-Musing conven-tional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonellaspp and Shigella spp isolates were MDR, respectively. The presence of CTX-M enzyme amongESBL-producing isolates was 85.18%, 77.7%, 50%, and 66.7%, in E. coli, Klebsiella spp, Salmonellaspp and Shhttp://irarticles.ir/wp-admin/post-new.php?post_type=download#igella spp respectively. The overall presence of CTX-M genes in Enterobacteriaceaewas 15.4% and among the resistant isolates was 47.6%. This study indicated that resistanceto -lactams mediated by CTX-M enzymes in Iran had similar pattern as in other parts ofthe world. In order to control the spread of resistance, comprehensive studies and programsare needed.© 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).