چکیده (انگلیسی):

Objective: To investigate the antioxidant/oxidant levels in blood serum and seminal
plasma of Arab stallion with different ages and their relation to semen quality.
Methods: Healthy Arabian stallions (n = 57), were divided into three groups. Young (5–
10years), Moderate (11–16 years) and Old stallions (>16 years) were subjected to semen
evaluation. Seminal plasma and blood samples were collected and stored at −20
C for
measuring glutathione reduced, nitric oxide, Malondialdehyde, ascorbic acid, copper and
zinc.
Results: Old stallions had significantly greater (P < 0.05) ejaculate volume, % live
sperm, and total sperm number compared to young and moderate aged groups. The
moderate age horses had significantly the lowest (P < 0.05) sperm concentration.
Compared to young horses, serum zinc concentrations of moderate and old horses were
significantly high (P < 0.0 001), but NO concentrations were significantly (P < 0.05) low.
Seminal plasma zinc, ascorbic acid and nitric oxide concentrations were significantly
(P < 0.05 and 0.01) high in young stallion group. No significant correlations were
observed between seminal zinc, copper, MDA and semen variables. Meanwhile, significant
negative correlations were observed between seminal plasma ascorbic acid concentration
and all semen variables except total sperm number and sperm abnormities %.
Significant correlations were observed between reduced glutathione and both of sperm
motility and % of live sperm. Nitric oxide concentrations correlated directly with individual
sperm motility but adversely with total sperm number.
Conclusion: Stallion age has significant effect on some semen variables, antioxidant/
oxidant status of either blood serum or seminal plasma.