چکیده (انگلیسی):

Background: Ves v 5 and Pol d 5, which constitute antigen 5, are recognized as the major, most potent
allergens of family Vespidae. Several studies have reported the diagnostic sensitivity of the novel recombinant
(r)Ves v 5 and rPol d 5 allergens in routine clinical laboratory settings by analyzing a group of
Vespula and Polistes venom-allergic patients. In this study, we analyzed the sensitivity to venom specific
(s)IgE by spiking with rVes v 5 and rPol d 5 in Japanese patients suspected of Hymenoptera venom
allergy.
Methods: Subjects were 41 patients who had experienced systemic reactions to hornet and/or paper
wasp stings. Levels of serum sIgE against hornet and paper wasp venom by spiking with rVes v 5 and
rPold d 5, respectively, as improvement testing, compared with hornet and paper wasp venom, as
conventional testing, were measured by ImmunoCAP.
Results: Of the 41 patients, 33 (80.5%) were positive (
0.35 UA/ml) for hornet and/or paper wasp venom
in conventional sIgE testing. sIgE levels correlated significantly (P < 0.01) between hornet (R ¼ 0.92) or
paper wasp venom (R ¼ 0.78) in improvement testing and conventional testing. To determine specificity,
20 volunteers who had never experienced a Hymenoptera sting were all negative for sIgE against these
venoms in both improvement and conventional testing. Improved sensitivity was seen in 8 patients
negative for sIgE against both venoms in conventional testing, while improvement testing revealed sIgE
against hornet or paper wasp venom in 5 (total 38 (92.7%)) patients.
Conclusions: The measurement of sIgE following spiking of rVes v 5 and rPol d 5 by conventional testing
in Japanese subjects with sIgE against hornet and paper wasp venom, respectively, improved the
sensitivity for detecting Hymenoptera venom allergy. Improvement testing for measuring sIgE levels
against hornet and paper wasp venom has potential for serologically elucidating Hymenoptera allergy in
Japan.