تولیدآنزیم نرخ خام زایش از آسپرژیلوس نیدولانس AKB-25بااستفاده از قسمت باقی مانده گرم سیاه بعنوان سوبسترا و استعمال صنعتی
Production of crude enzyme from Aspergillus nidulans AKB-25 using black gram residue as the substrate and its industrial applications
نویسندگان |
این بخش تنها برای اعضا قابل مشاهده است ورودعضویت |
اطلاعات مجله |
Journal of Genetic Engineering and Biotechnology (2016).volume 14 |
سال انتشار |
2016 |
فرمت فایل |
PDF |
کد مقاله |
9647 |
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چکیده (انگلیسی):
The production of crop residues in India is estimated to be about 500–550 million tons
annually. It is estimated that about 93 million tons of crop residues is burnt annually which is not
only wastage of valuable biomass resources but pollution of the environment with the production of
green house gases also. Among different low cost crop residues, black gram residue as the substrate
produced maximal endoglucanase, FPase, and b-glucosidase activities from Aspergillus nidulans
AKB-25 under solid-state fermentation. During optimisation of cultural parameters A. nidulans
AKB-25 produced maximal endoglucanase (152.14 IU/gds), FPase (3.42 FPU/gds) and xylanase
(2441.03 IU/gds) activities. The crude enzyme was found effective for the saccharification of pearl
millet stover and bio-deinking of mixed office waste paper. The crude enzyme from A. nidulans
AKB-25 produced maximum fermentable sugars of 546.91 mg/g from alkali-pretreated pearl millet
stover by saccharification process at a dose of 15 FPU/g of substrate. Pulp brightness and deinking
efficiency of mixed office waste paper improved by 4.6% and 25.01% respectively and mitigated dirt
counts by 74.70% after bio-deinking. Physical strength properties like burst index, tensile index and
double fold number were also improved during bio-deinking of mixed office waste paper.
2016 Production and hosting by Elsevier B.V. on behalf of Academy of Scientific Research &
Technology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/
licenses/by-nc-nd/4.0/).
کلمات کلیدی مقاله (فارسی):
قسمت باقی مانده گرم سیاه.انزیم های پروتئین شکن توده سلولی.اسپرژیلوس نیدولانس.قندسازی .خواربار جو ارزن کوبیده .
کلمات کلیدی مقاله (انگلیسی):
Black gram residue; Biomass-degrading enzymes; Aspergillus nidulans; Saccharification; Pearl millet stover; Bio-deinking
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