چکیده (انگلیسی):

A binary mixture of almitrine besmesylate (A) and raubasine (R) was determined by two
different chromatographic methods. The first method was based on HPTLC separation of the two
drugs followed by densitometric measurement of their spots at 245 and 285 nm for A and R, respectively.
The separation was carried out using HPTLC silica gel F254 nanoplates with methanol:
ammonia (10:8, v/v) as developing solvent. The linearity was achieved over concentration
range of 0.5–8 lg/spot and 0.5–10 lg/spot with mean accuracy 100.79± 1.58 and 100.68± 1.78,
for A and R, respectively. The second method involved the determination of A and R using reversed
phase high performance liquid chromatography (HPLC) on C18 column using acetonitrile:potassium
dihydrogen orthophosphate buffer pH= 4.7 (70:30, v/v) as mobile phase with flow rate at
2 ml/min. Quantitation was achieved using UV detection at 220 nm. A linear relationship was
obtained over a concentration range of 0.75–105 lg ml1 for both drugs with mean accuracy
100.85± 1.74 and 98.82 ±1.31, for A and R, respectively. The methods were successfully applied
for the determination of the cited drugs in dosage forms. The proposed methods were validated
according to USP and were found to be valid and suitable for the assay of the cited drugs in dosage
forms in quality control laboratories.