چکیده (انگلیسی):

The purpose of this study was to design an innovative topical ointment containing
betamethasone dipropionate loaded nanostructured lipid carrier (BD-NLC) for the treatment
of atopic dermatitis (AD). BD-loaded NLC was produced with precirol ATO 5 and oleic
oil (OA) by melt emulsification method. Effects of surfactant concentration, amount of solid
lipid and liquid lipid on skin retention and skin penetration were investigated by in vitro percutaneous
permeation experiment. The optimized BD-NLC showed a homogeneous particle
size of 169.1 nm (with PI = 0.195), negatively charged surface (−23.4 mV) and high encapsulation
efficiency (85%). Particle morphology assessed by TEM revealed a spherical shape. In
vitro skin permeation study was carried out to investigate the percutaneous behaviors of W/O
ointment with BD-NLC and Carbopol emulgel ointment with BD-NLC.W/O ointment with BDNLC
showed high skin retention (35.43 μg/g) and low penetration (0.87 μg/ml). In vitro drug
release studies were carried out to demonstrate the drug releasing properties of the two ointments.
W/O ointment with BD-NLC showed an advantage for skin retention as it was better
for drug release. The tissue distribution test suggested that BD distribution was
skin >muscle > blood. Self-made topical ointment in mice showed no skin irritation.The animal
experiments indicated that BD-loaded NLC ointment was effective and safe for topical use.