تنظیم منفی حالت ایمنی کلاودین ۷ در سرطان بدخیم سلول های اپیتلیال مثانه
Down-regulated claudin-7 immunoexpression in urothelial carcinoma of the urinary bladder
نویسندگان |
این بخش تنها برای اعضا قابل مشاهده است ورودعضویت |
اطلاعات مجله |
Arab Journal of Urology www.journals.elsevier.com/arab-journal-of-urology |
سال انتشار |
2013 |
فرمت فایل |
PDF |
کد مقاله |
5213 |
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چکیده (انگلیسی):
Objectives
To analyse the gene-expression level of claudin-7 in urothelial carcinoma (UC) of the urinary bladder, and its relationship with clinicopathological variables.
Materials and methods
This study included 68 specimens of UC of the bladder, comprising 35 with non-muscle-invasive (NMI), stage Ta–T1, and 33 with muscle-invasive (MI) tumours, T2–T4, and 26 of normal urothelium (NU). Total RNA was extracted and 1 μg was reverse transcribed using a cDNA kit. RT-PCR was conducted using SYBR Green I dye to examine the expression levels of the target gene (claudin-7) and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase. Using confocal-laser scanning light microscopy, immunohistochemistry (IHC) was used to validate the RT-PCR data. The correlation between claudin-7 and the clinicopathological variables was assessed.
Results
Claudin-7 was down-regulated in UC samples compared to NU samples (P < 0.001). NMI (Ta–T1) tumours had significantly higher claudin-7 expression than MI (⩾pT2) tumours (P = 0.012). There was no significant difference between patients with G1-2 tumours and those with G3 tumours (P = 0.19). There was no significant difference between patients with recurrent NMI UC and those with no recurrence (P = 0.61). IHC showed a lower expression of claudin-7 in the UC samples than NU samples, and in MI UC than in NMI UC.
Conclusions
These results indicate that a reduced expression of claudin-7 correlates with the invasiveness and progression of UC of the urinary bladder. Further studies are needed to validate claudin-7 as a marker for UC.
کلمات کلیدی مقاله (فارسی):
سرطان مثانه; کلاودین 7; پی سی ار بهنگام; پروتیین های اتصال محکم; سرزان بدخیم اپیتلیال ادراری
کلمات کلیدی مقاله (انگلیسی):
Bladder cancer; Claudin-7; Real-time PCR; Tight junction proteins; Urothelial carcinoma
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