چکیده (انگلیسی):

Objective: To evaluate the histopathological changes in ovarian and uterine tissues
associated with oxidative stress induced by electromagnetic waves.
Methods: Thirty female Sprague Dawley of 180 g body weight and 3 months old were
used in the experimental work. Animals were divided into control and two experimental
groups. Experimental groups were exposed to 1800 MHz Global System for Mobile
Communication radiofrequency radiation emitted by a signal generator for 2 h per day for
30 and 60 d, respectively. Following exposure, serum, ovaries and uteri were collected for
biochemical and histopathological investigations.
Results: Biochemical analysis showed alterations in oxidative stress parameters in both
ovaries and uteri tissues in comparison to control group. The histopathological changes
were more prominent in experimental groups, in the ovary were included vacuolation in
interstitial, granulosa, luteal cells and ooplasm. Other histopathological changes are
disorientation of corona radiata, disruption and thinning of the zona pellucida. Cellular
nucleus changes similar to fragmentation of the nucleus indicate the start of a degeneration
process at Graafian follicles as well as micronuclei formation in oocyte nucleus and
in some luteal cells. Histopathological changes in uterine tissue confined to increase
height of luminal epithelium cells, sever apoptosis of glandular and luminal epithelium
cells, and sever eosinophils, polymorphonucleocyte lymphocytes and macrophage's
infiltration in myometrium and endometrium layers. Vascular congestion points out for
the existence of inflammatory response changes in the endometrium.
Conclusion: Results executed that the potential alteration of antioxidant capacity may
contribute to endometrial oxidative damage that could be related to pathogenesis and
progression of endometritis.