چکیده (انگلیسی):

Objective: To study the effect of sitagliptin on adipocytokines expression in diabetic rats
and its molecular mechanism.
Methods: Male SD rats were chosen and randomly divided into NC group, T2DM
group, SP group and SP + LY group. NC group received conventional breeding, T2DM
group, SP group and SP + LY group received intraperitoneal injection of streptozotocin
after 12 weeks of high-fat diet to establish diabetes animal model, SP group received
sitagliptin intervention and SP + LY group received sitagliptin combined with PI3K
inhibitor LY294002 intervention. Six weeks after the intervention, serum was collected to
determine the levels of biochemical indexes and adipocytokines, and visceral adipose
tissue was collected to determine expression levels of adipocytokines.
Results: Serum TC, TG, LDL-C, FBG, FINS, Leptin and Chemerin levels as well as
HOMA-IR of T2DM group were higher than those of NC group, and HDL-C, Adiponectin
and Omentin-1 levels were significantly lower than those of NC group; serum TC,
TG, LDL-C, FBG, FINS, Leptin and Chemerin levels as well as HOMA-IR of SP group
were lower than those of T2DM group, and HDL-C, Adiponectin and Omentin-1 levels
were significantly higher than those of T2DM group; Leptin and Chemerin levels in
serum and visceral adipose tissue of SP + LY group were higher than those of SP group
while Adiponectin and Omentin-1 levels were significantly lower than those of SP group.
Conclusion: Sitagliptin can regulate the expression of adipocytokines in adipose tissue
of diabetic rats through PI3K-AKT pathway.