چکیده (انگلیسی):

A simple chemometrics-assisted spectrophotometric method for the simultaneous determination
of lamivudine and stavudine in pharmaceutical tablets is described. TheUVabsorption spectra
of the studied drugs, in the range of 200–310 nm, showed a considerable degree of spectral overlapping
([Di]0.5= 94.9%). Resolution of the mixture has been accomplished by using classical least-squares
regression analysis (CLS) and principle components regression analysis methods (PCR). Beer’s law
was obeyed for both drugs in the general concentration ranges of 2–12 and 3–15 lg ml1 for lamivudine
and stavudine, respectively. The proposed methods were successfully applied for the determination
of the two drugs in laboratory prepared mixtures. The overall recoveries percent were found
98.58±1.53–101.30±1.35 (CLS) and 98.62±1.65–101.13± 1.04 (PCR) for lamivudine and
98.43±1.62–99.42± 1.55 (CLS) and 98.23± 1.97–101.20±1.79 (PCR) for stavudine, respectively.
The commercial tablets percentage content was found 98.10±2.5–102.47±2.94 (CLS) and
99.12±1.71–100.92±1.54 (PCR) for lamivudine and 96.00±2.94–98.17± 1.72 (CLS) and
97.40±1.55–97.80± 1.92 (PCR) for stavudine, respectively. Good percentage recoveries and proper
statistical data obtained with both the laboratory prepared mixtures and the commercial tablets
proved the suitability and efficiency of the proposed procedures for routine analysis and quality control
purposes with quite satisfactory precision. A comparison of the obtained results from CLS and PCR
were also performedwith those obtained from reported method. The obtained F- and t-values obtained
indicating no significant differences between the results of the proposed and reported methods.