چکیده (انگلیسی):

A high performance liquid
chromatography with tandem mass spectrometry
(LC-MS/MS) method was developed to measure the
thymosin alpha 1 (Ta1) concentration in human
serum. Ta1 in human serum was determined by
solid phase extraction and reverse phase LCMS/
MS. The high-performance liquid
chromatography (HPLC) system interfaced with the
MS/MS system with a Turbo Ion spray interface.
Positive ion detection and multiple reaction
monitoring (MRM) mode were used for this human
serum quantitation. Eight different concentration
standards were used to establish the detection range.
Six quality control (QC) and 2 matrix blanks were
checked by calibration curves performed on the
same day. The lower quantitation limit was 0.5
ng/mL T a1 in human serum. Calibration curves
were established between 0.5 to 100 ng/mL by
weighted linear regression. The correlation
coefficients for different days were 0.9955 or
greater. Quantitation of Ta1 by the LC-MS/MS
method is fast, accurate, and precise.