چکیده (انگلیسی):

A specific, sensitive and rapid method based on high performance liquid chromatography
(HPLC) was developed for the simultaneous determination of telmisartan (TELM) and hydrochlorothiazide
(HCT) in human plasma using indapamide as internal standard. The method utilizes
proteins precipitation with acetonitrile as only sample preparation prior to RP-HPLC.
The analytes were chromatographed on shim-pack cyanopropyl column in isocratic elution with
methanol: 10 mM ammonium acetate solution (pH 6.0) (35:65 v/v) as mobile phase at a flow rate
of 1 ml/min and the wavelength of detection was 270 nm.
The method was validated over the concentration range of 1–10 lg ml1 for TELM and 0.31–
3.12 lg ml1 for HCT in human plasma.
Inter- and intra-run precision of TELM and HCT were less than 3.60% and the accuracy was less
than 1.868%. The linearity, recovery, matrix effect and stability were validated for TELM/HCT in
human plasma.