چکیده (انگلیسی):

Objective: To determine the expression of non-muscle myosin heavy chain 9 (MYH9) in
osteosarcoma and its effect on the migration and invasion abilities of tumor cell.
Methods: A total of 65 cases of osteosarcoma and 20 cases with benign osteochondroma
who underwent resection operation in the Orthopaedics Department of our hospital from
January 1st 2009 to January 1st 2015 were selected. Their mRNA levels of MYH9 were
tested by qrt-PCR. Immunohistochemical method was used to examine the expression of
MYH9 in osteosarcoma and the correlation between the positive expression of MYH9
and the clinicopathological features of patients was illustrated by statistical analysis.
MYH9 was compounded artificially. The expression of MYH9 in SAOS2 osteosarcoma
cells was decreased by siRNA. Scratch test was used to determine the change of SAOS2
cell migration ability after MYH9 silence. Transwell assay was employed to detect the
change of cell invasion ability after MYH9 silence.
Results: The expression levels of mRNA of MYH9 and protein in osteosarcoma tissues
were significantly higher than those in benign osteochondroma tissues. The high
expression of MYH9 in osteosarcoma tissues was apparently related to the high Enneking
classification (III classification) and lung metastasis. SiRNA of MYH9 could evidently
decrease the expression level of MYH9 in SAOS2. The down-regulated expression of
MYH9 could inhibit the migration and invasion abilities of SAOS2 cells.
Conclusions: MYH9 shows a trend of high expression in osteosarcoma tissues, and its
high expression is associated with features such as tumor invasion and metastasis. The
down-regulated MYH9 can realize an anti-tumor effect by inhibiting the migration and
invasion of osteosarcoma cells.