چکیده (انگلیسی):

In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, andColeoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individuallytested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera:Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a(+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3)strain rendered competent with CaCl2. Isopropyl -d-1-thiogalactopyranoside was used toinduce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Abwas obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 g g−1caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. How-ever, the mortality rates of Bt SY49-1 strains’ spore–crystal mixture at the same dose wereobserved to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicatedthat cry1Ab may be considered as a good candidate in transgenic crop production and as analternative biocontrol agent in controlling stored product moths.© 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).