چکیده (انگلیسی):

Butyricimonas phoceensis strain AT9 (= CSUR P2478 = DSM
100838 was isolated from the faeces of a 57-year-old French
woman living in Marseille with class III morbid obesity (body
mass index (BMI) 55.8 kg/m2). This isolate is part of an
exploratory study of the gut flora from obese patients before
and after bariatric surgery. Bariatric surgery is the most effective
treatment for morbid obesity for sustainable weight loss
and leads to an enrichment of the gut flora [1]. The goal of our
study was to compare microbial diversity of the gut flora in
obese patients before and after bariatric surgery by culturomics.
The aim of culturomics is to exhaustively explore the
microbial ecosystem of gut flora by using different culture
conditions followed by matrix-assisted laser desorption/ionization
time-of-flight mass spectrometry (MALDI-TOF MS)
identification [2].
The conventional approaches used in the delineation of
bacterial species are 16S rRNA sequence identity and phylogeny
[3], genomic (G+C content) diversity and DNA-DNA hybridization
(DDH) [4,5]. However, these approaches present
some difficulties, mainly as a result of their cutoff values, which
change according to species or genera [6]. The accession of
new technology tools, such as high-throughput sequencing, has
made available nucleotide sequence libraries for many bacterial
species [7]. We recently suggested incorporating genomic data
in a polyphasic taxonogenomics approach to describe new
bacteria. This procedure considers phenotypic characteristics,
genomic analysis and the MALDI-TOF MS spectrum comparison
[8,9].
Here we propose a classification and a set of characteristics
for Butyricimonas phoceensis strain AT9, together with the
description of complete genome sequencing, annotation and
comparison as new species belonging to the genus Butyricimonas.