چکیده (انگلیسی):

Objective: To determine Blastocystis frequency and subtypes (ST) in ulcerative colitis
(UC) patients and analyse some laboratory findings between Blastocystis positive and
negative cases.
Methods: Faecal samples from 150 UC patients in Adnan Menderes University,
Training and Research Hospital were examined by direct microscopy and cultivated in
Jones medium. Blastocystis positive cultures were subjected to DNA isolation and subtypes
were identified by sequencing of barcode region. A retrospective analysis was
conducted on C reactive protein (CRP), leucocyte counts (WBC), neutrophil counts, and
sedimentation rates.
Results: The overall positive rate of Blastocystis was 8% (12 patients) and the most
abundant subtype was ST3 (eight isolates, 66.7%), followed by ST1, ST2 and ST7.
Laboratory findings between Blastocystis infected and non-infected UC patients were not
significantly different. Blastocystis frequency was 3.8% among the patients in active
stage, while it was 11.8% among the patients in remission stage.
Conclusions: The present study confirms previous findings that have indicated the predominance
of Blastocystis ST3 in humans and contributes additional evidence that suggests
the low colonisation of Blastocystisinfection in ulcerative colitis patients during active stage