چکیده (انگلیسی):

Objective: To study the effect and mechanism of the dysfunction of CD4+ T cells in the
disease process of chronic cardiac failure (CHF).
Methods: According to different group technologies, 100 CHF patients were divided
into the following groups: ischemia group and non-ischemia group, heart function Ⅲ–Ⅳ
group and heart function Ⅰ–Ⅱ group, event group and non-event group, and 50 healthy
volunteers were included in the control group. Real-time PCR was used to detect transcription
factors T-bet and GATA-3 of Th1 and Th2; flow cytometry was applied to
determine the ratio of Th17 and Treg cells; ELISA was employed to test cytokines IFN-g,
IL-4, IL-17 and IL-10 of peripheral blood Th1, Th2, Th17 and Treg cells, respectively;
ultrasonic cardiogram was used to exploit to LVEF and LVEDd; and electrochemilu
minescene immunoassay was used to examine plasma BNP. The differences of all indexes
of all groups were analyzed and the correlation between CD4 T cells and clinical
indexes was analyzed by Pearson correlation analysis.
Results: As compared to the control group, the transcription factors T-bet and GATA-3
of Th1 and Th2, the ratio of cytokines Th17 and IFN-g, cytokines IL-17, T-bet/GATA-3,
IFN-g/IL-4, Th17 cells/Treg cells, IL-17/IL-10 of the ischemia group and non-ischemia
group, heart function Ⅲ–Ⅳ group and heart function Ⅰ–Ⅱ group, event group and nonevent
group were all increased significantly, while their transcription factor GATA-3
of Th2, cytokines IL-4, Treg cells ratio, cytokines IL-10 were decreased obviously.
The differences showed statistical significance (P < 0.05). The increase or decrease of the
partial CD4+ T cells of the ischemia group, heart function Ⅲ–Ⅳ group and event group
was more distinctly. The results of Pearson correlation analysis showed that IFN-g and
IL-17 were significantly positively correlated with LVEDd and BNP, IL-4 and IL-10
were also significantly positively correlated with LVEF, but correlated negatively with
BNP, and IL-17 was negatively correlative with LVEF.
Conclusions: There was a correlation between CHF and the dysfunction of CD4+ T cells
showing immune activation phenomenons of deviations from the Th1/Th2 balance towards
Th1 and from the Th17/Treg balance towards Th17, which was also related to the
types, severity and prognosis of the disease.