چکیده (انگلیسی):

Objective: To investigate suitable condition for extraction of the active components
from Ajuga nipponensis (A. nipponensis).
Methods: Orthogonal experimental design was used to determine the optimal extraction
parameters for ecdysterones and flavonoids. Finally, the hepatoprotective abilities of
A. nipponensis extracts were evaluated by CCl4-induced animal models.
Results: Maximum yields of flavonoids (7.87 ± 0.10) mg/g and ecdysterones
(0.73 ± 0.02) mg/g could be obtained when the extraction time was 50 min, the
extraction temperature was 60
C, and the ratio of sample to 70% (v/v) ethanol was
1:20 (w/w). The antioxidant property of A. nipponensis was correlated to the concentration
of its extracts. At 5 mg/mL, A. nipponensis extract scavenged 84.8% of DPPH
radical and had absorbance values of 2.43 ± 0.04 reducing power. Upon CCl4-induced
liver injury, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase
decreased significantly after the mice were treated with A. nipponensis. Histological
researches also explained that A. nipponensis reduced the extent of liver lesions induced
by CCl4.
Conclusions: A. nipponensis exhibited potent antioxidant activity in chemical experimental
models and hepatoprotective effect against CCl4-induced liver damage.