چکیده (انگلیسی):

Objective: To find out a novel therapy based on mobilization and differentiation of own
body-derived stem cells using natural bee honey.
Methods: Testicle degeneration model of mouse is available through food fasting 5 days
long that causes malnutrition and affects the testicle. Then administration of 30% (T1)
and 50% bee honey (T2) each for five days long and compared to the positive control, fast
without bee honey (T0+) and negative control, feed but without bee honey (T0−). Subsequently
observation of Hematopoietic Stem Cells (HSCs) mobilization according to the
CD34 and CD45 expressions performed using flowcytometry method was conducted.
Besides, identification of differentiation is examined through the expression of spermatogonial
stem cells (SSCs) using immunohistochemistry technique, while the seminiferous
tubules cell regeneration through H&E staining microscopic examination.
Results: Stem cells mobilization based on the expression of CD34 and CD45, which is a
marker of HSCs. Differentiation of stem cells into progenitor cells that expected based
expression of SSCs in testicle tissue. SSCs increase causes regeneration testicle seminiferous
tubules.
Conclusions: Results of this study revealed a significantly different of C34 and CD45
expressions between groups, also an increase SSCs expression and testicle seminiferous
tubules cells regeneration as well.