چکیده (انگلیسی):

Objective: To study the regulatory effects of miR-21 on breast cancer cell line proliferation
and invasion as well as the downstream target genes.
Methods: Breast cancer cell lines MCF-7 were cultured and transfected with miR-21
mimics and the corresponding negative control mimics (NC mimics), and then MTS
kits were used to detect cell viability. Transwell experiment was used to detect cell invasion
ability, and fluorescence quantitative PCR was used to detect the expression of
proliferation and invasion-related genes in cells.
Results: 24 h after transfection of miR-21 mimics and NC mimics, cell OD value and the
number of invasive cells of miR-21 group were significantly higher than those of NC
group, and mRNA contents of PDCD-4, FasL, PTEN, RhoB, Maspin, TIMP3 and RECK
in cells were significantly lower than those of NC group.
Conclusion: miR-21 can promote the proliferation and invasion of breast cancer cell
lines, and its downstream target genes include PDCD-4, FasL, PTEN, RhoB, Maspin,
TIMP3 and RECK.