چکیده (انگلیسی):

Background: Chronic rhinosinusitis (CRS) is characterized by local inflammation of the sinonasal tissues.
CRS patients with nasal polyps and asthma often develop acute exacerbation of sinonasal symptoms after upper
respiratory tract infections. However, the influence of concomitant asthma on the nasal immune response
to viral infection remains unclear.
Methods: Specimens of nasal polyp and mucosal tissues were obtained from 3 groups of CRS patients (n =
14 per group): 1) patients without asthma (CRS group), 2) patients with aspirin-tolerant asthma (ATA group),
and 3) patients with aspirin-intolerant asthma (AIA group). Nasal fibroblasts isolated from the specimens were
stimulated with poly I:C. CXCL10 expression was analyzed by the quantitative real-time polymerase chain reaction
and enzyme-linked immunoadsorbent assay. Biopsy specimens from CRS patients without asthma were
subjected to immunohistochemistry for detection of T-bet and GATA-3 expression in CD3+ T cells by double labeling.
Results: Nasal fibroblasts from the ATA and AIA groups showed significantly enhanced expression of CXCL
10 mRNA and protein after poly I:C stimulation compared with cells from the CRS group and the control group
(normal nasal mucosa). In addition to T helper (Th)2 cells, there was more abundant infiltration of Th1 cells into
tissues from the AIA and ATA groups.
Conclusions: Our findings suggest that CRS associated with asthma may become intractable through the
over-production of CXCL10 in response to viral infection.